The expression levels of the four hub genes were verified in the TCGA-BLCA dataset and the GEO dataset (Figs. S3A,D, S4), CDH19, RELN, and PLP1 levels in normal bladder tissues were significantly higher than those in BC tissues, while the expression of TRIB3 was higher in BC tissues than in normal bladder tissues. The gene discussed is TRIB3; the disease is breast cancer.