As shown in Fig. 4C, the top 20 genes with the highest scores in the tumour group, including KIF2C, NUSAP1, MELK, PBK, KIF20A, AURKA, NCAPG, TPX2, KIF4A, ASPM, AURKB, CDC20, CCNB1, BUB1B, CCNB2, CCNA2, BUB1, TOP2A, UBE2C, and TTK, and the top 11 genes with the highest scores in the normal group, including GPM6B, CYP2U1, SNCA, PLP1, LGI4, RELN, MPZ, CDH19, GFRA3, COBL, and SNAP25, were identified as hub genes. This evidence concerns the gene BUB1 and neoplasm.