These KO mice, recently used to characterize the pro-inflammatory role of Serpinb3 in murine NASH [13], were fed for this part of the study on choline-deficient aminoacid-defined (CDAA) dietary protocol to induce experimental NASH, very effective and reliable to induce steatosis, inflammatory response and fibrosis [12,13,23]. Here, SERPINB3 is linked to metabolic dysfunction-associated steatohepatitis.