Previous studies have reported that pharmacological blockade of PAR2, via pepducin, a short peptide derived from a GPCR intracellular loop, effectively suppressed NASH features in mice fed with MCD diet, although the mechanistic basis of the PAR2-promoting effect on liver steatosis in the MCD model was not yet known [44]. This evidence concerns the gene F2RL1 and metabolic dysfunction-associated steatohepatitis.