The following conclusions were obtained from multivariate regression analysis for the independent risk variables for GC development (P < 0.05, Table 3, Fig. 3A): The multivariate regression analysis revealed that age (P = 0.0018), tumor size (P = 0.046), Lauren type (P = 0.003), TNM stage (P = 0.0045), and histological grade (P = 0.01) and increased expression of PLCD3 (P = 0.001). This evidence concerns the gene PLCD3 and gastric cancer.