Furthermore, increased expression of proinflammatory cytokines such as IL-6, IL-1β, and TNFα that we detected in IFNγ treated SPG11 iMGL, was also described for iPSC-derived microglia from patients with other neurodegenerative diseases, including PD and ALS [2, 4, 57, 68]. Here, TNF is linked to amyotrophic lateral sclerosis.