FC analysis of monocyte subsets revealed increased levels of classical (CD14++CD16−) and intermediate (CD14++CD16+) monocytes in SPG11–HSP patients (n = 8) compared to controls (n = 38), while the percentage of non-classical (CD14low/+CD16++) monocytes was unchanged (Fig. 3a, b; Online Resource Fig. 3). Here, SPG11 is linked to hereditary spastic paraplegia.