In this study, we identified eight GRTTKs associated with the development and prognosis of ESCC including EIF4H, CDK2, TCEA1, SPTLC2, TMEM209, RGP1, CAPZA3 and EIF3D based on the transcriptional matrix profiling analysis of TCGA and GEO cohorts. The gene discussed is TMEM209; the disease is esophageal squamous cell carcinoma.