Here, we characterized the expression levels of m1A methyltransferase complex TRMT6 and TRMT61A, as well as the m1A demethylases ALKBH1 and ALKBH3 in five BLCA cell lines of different BLCA grades and stages and in a control cell line derived from a healthy donor. This evidence concerns the gene TRMT61A and bladder transitional cell carcinoma.