EHHADH and hereditary clear cell renal cell carcinoma: RCC4 cells are derived from human renal cell carcinoma and are VHL-mutated (29) qPCR and Western blot analysis were performed to compare EHHADH mRNA and protein expression between VHL-mutated RCC4 cells (controls) and an RCC4 subline reconstituted with VHL, here referred to as RCC4+VHL cells.