The methylation levels of DMRs in ZBTB38, FMN1, ALDH3B2, KAZN, KCNQ1, TNNT3, and ELF1 genes were significantly lower in HCC cell lines, while those in SULT2B1, SLC6A19, LINC01550, PNKD, and TPM4 genes did not show significant differences (Figs. 2, 3, 4, 5, Supplementary Fig. 2). The gene discussed is ALDH3B2; the disease is hepatocellular carcinoma.