To identify the relationship between TACSTD2 promoter hypermethylation and TROP2 protein expression, we first determined the TROP2 expression status in seven different colon cancer cell lines (DLD1, LoVo, LS-174T, SW480, HT29, SW620, and HCT116) and one rectal cancer cell line (SW837) by western blotting. The gene discussed is TACSTD2; the disease is malignant colon neoplasm.