Although the levels of acylation patterns of ceramide, SPH and S1p were not significantly different in tumor tissues of WT PyMT mice versus NcDase−/− PyMT mice (Supplementary Fig. 7d), the levels of C18, C18:1, C24, and C24:1 ceramide species were higher in NcDase-deficient TAMs from MMTV-PyMT cancer models (Fig. 7e). The gene discussed is ASAH2; the disease is cancer.