In addition, to further verify whether the four proteins (COL12A1, THBS2, S100A8, and S100A9) showed consistent up-regulation in the tissue samples respectively from CRC patients with RAS mutation and no metastases, we also searched the public cohort from the CPTAC, which composed of a total of 96 tumor and matched normal adjacent tissues (NATs) pairs from CRC patients38. This evidence concerns the gene THBS2 and colorectal carcinoma.