When the differential expression was dropped from 2-fold to 1.5-fold change, there were still only the four proteins (COL12A1, THBS2, S100A8, and S100A9) were screened among the three independent cohorts (Supplementary Fig. 2A), indicating the four proteins could be used as the potential biomarkers to distinguish CRC patients from healthy controls. The gene discussed is S100A9; the disease is colorectal carcinoma.