Under pathological conditions, TDP43 accumulates in neurons and consumes TDP43 protein expression through the above loop, mRNA levels significantly increase, leading to larger aggregates carrying more newly synthesized TDP43, leading to erroneous AS of genes involved in neuromuscular junctions,405 where MacNair et al.406 used translating ribosome affinity purification (TRAP) technology to find that MTHFSD and DDX58 regulated by TDP43 affect ALS-related neuroinflammation and stress granule pathways. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.