Strikingly, four of them, ornithine transcarbamylase (OTC), carbamoyl phosphate synthetase 1 (CPS1), argininosuccinate synthetase 1 (ASS1), and N-acetylglutamate synthase (NAGS) (Fig. 2J) are enzymes involved in the urea cycle, a metabolic route responsible for ammonia detoxification impaired in NAFLD experimental models and patients, as a result of hepatic fatty acid accumulation [25], contributing to development of hepatic fibrosis [26]. Here, CPS1 is linked to metabolic dysfunction-associated steatotic liver disease.