Although exploring aging-dependent metabolic effects resulting from Acod1 deficiency are beyond the aim of the present work, our results demonstrate that Acod1 plays no major roles in regulating body weight, fat depots, glycemic control, liver steatosis and obesity-associated gut microbiota composition in mice fed with normal chow for ~5 months. Here, ACOD1 is linked to obesity due to melanocortin 4 receptor deficiency.