To further reveal the effect of FSTL3 on tumor growth and tumor immunity of CRC, immunocompetent and immunodeficient mouse tumor models were constructed by subcutaneously implanting FSTL3-KD or NC-KD MC38 cells, which were transfected with lentivirus-mediated shFSTL3 or shNC (Supplementary Fig. S6A). The gene discussed is FSTL3; the disease is neoplasm.