To further evaluate the impact of high FSTL3 expression on ICB therapy, mouse subcutaneous tumor models were constructed with NC-OE and FSTL3-OE MC38 cells, which were transfected with lentivirus-mediated control cDNA and FSTL3Flag cDNA (Supplementary Fig. S6A), followed by anti-PD1 therapy. This evidence concerns the gene PDCD1 and neoplasm.