To further validate ISM1 ameliorated cardiomyocyte injury through stimulating SIRT1 O-GlcNAcylation in vitro, D-gal-induced H9C2 cells with AdhISM1 infection were treated with alloxan monohydrate (ALX), an OGT inhibitor, as shown in Supplementary Figure 5, the increased SIRT1 activity was counteracted by ALX. This evidence concerns the gene ISM1 and infection.