GSDME and neoplasm: In the 4T1 tumor-bearing mice, a mild photothermal effect at 45°C can induce ICD and activate an immune response, thereby activating CD8+ T cells to secrete PFR to pierce the plasma membrane of tumor cells, promoting RNase A entry into tumor cells, and activating caspase-3 and gasdermin-E pathways to induce apoptosis and scorch death to activate immunity further.