To determine the mode of cell death involved in the reduction of osteosarcoma cell viability by TGT, we treated 143B and SAOS2 cells with TGT at concentrations of 0, 25, 50, and 75 mg/mL, followed by staining using APC Annexin V in combination with 7-AAD. The gene discussed is ANXA5; the disease is osteosarcoma.