Moreover, we screened out the specifically amplified fragments (TRBV27, TRBV28, TRBV3-2, and TRBJ1-6), and also 25 differentially expressed CDR3 sequences (8 up-regulated and 17 down-regulated) in the complication group, which had the potential to be a marker to predict complications of T2D patients. Here, TRBV20OR9-2 is linked to type 2 diabetes mellitus.