Here, we aimed to independently recreate representative KCNH2 N588D (LQTS) and N588K (SQTS) mutations in the genomic background of 409B2 hiPSCs31, serving as an isogenic baseline for several mutations, since this line is well-established and previously showed high cardiac differentiation potential32,33. This evidence concerns the gene KCNH2 and Familial short QT syndrome.