Collagen networks were imaged using second harmonic generation (SHG) with two-photon microscopy in liver samples from humans (healthy control, NASH, NASH + T2DM; Extended Data Fig. 3a), as well as in mice (chow, HiAD, HiAD + PM, HiAD + ALT-711 and in AAV8-AGER1-injected livers; Extended Data Fig. 3b). The gene discussed is DDOST; the disease is metabolic dysfunction-associated steatohepatitis.