SMAD2 and Hepatic fibrosis: There were still few studies on the role of Robo protein in chronic liver diseases such as liver fibrosis: Yuen et al.19 found that Robo1 was involved in the pathogenesis and progression of renal fibrosis in mice with ischemia and obstructive renal injury; in addition, Chang et al.20 verified that Robo1 expedited liver injury and liver fibrosis by stimulating HSCs in CCl4-induced mouse liver fibrosis model, and they further proved that inhibiting Slit2-Robo1 signaling pathway suppressed the activation of HSCs by blocking Smad2/3 and PI3K/Akt signaling pathways.