IL33 and neoplasm: To assess whether Pik3cg gene-edited macrophages could maintain their immunostimulatory phenotype in an immunosuppressive microenvironment, we utilized 4T1 tumor cell supernatant rich in immunoregulatory cytokines (TGF-β, IL-33, IL-4 and IL-10) to mimic the in vivo tumor setting (Supplementary Fig. 21a)22.