The places we would expect colocalization of these two markers are at sites where an Iba1-positive cell (macrophage, microglia) (74) and a GFAP-positive cell (astrocyte, glioma) touch (75), or where microglia have phagocytosed GFAP-containing fragments (75), or possibly a cell type with a dual astrocytic and macrophage/microglia molecular phenotype (76–79). Here, GFAP is linked to glioma.