dExPath images yielded expected biological targets such as putative neurons (via anti-MAP2 antibody), neurofilaments (via anti-NF-L), AD plaques (via anti-β-amyloid), and PD aggregates (via anti-α-synuclein), but demonstrated better resolution, especially for densely-packed filamentous structures such as MAP2 and NF-L, and more structural detail with amyloid plaques and α-synuclein aggregates, vs. standard DAB analysis. Here, NEFL is linked to Parkinson disease.