We evaluated in murine tumor cell lines B16F10, 4T1, and CT26 whether electrochemotherapy triggered changes in immunogenic cell death DAMPs: calreticulin, ATP, high mobility group box 1 (HMGB1), and four immunologically important cellular markers: MHCI, MHC II, PD-L1 and CD40. The gene discussed is HMGB1; the disease is neoplasm.