Pharmacological inhibition of either IRE1α or NF-κB by STF-083010/ MKC8866 and BAY-11-7085 respectively could significantly suppress TG or HA15-induced increased transcription and secretion of both IL-6 and TNF-α in both A2058 and A375 melanoma cell lines (Fig. 4G-H; Additional file 1: Fig. S6L-M). This evidence concerns the gene TNF and melanoma.