Our in vitro CCK8 and immunoblotting analysis have revealed that HA15 treatment for 24 h could suppress the cell viability of B16F10 melanoma cells in a dose-dependent manner (Additional file 1: Fig. S2A), and 10 μM HA15 treatment triggered prominent activation of IRE1α branch as revealed by the up-regulation of phosphor-IRE1α and spliced XBP1 (XBP1s) (Fig. S2B), indicating HA15 works properly to induce ER stress and thereby the activation of IRE1α signaling. The gene discussed is XBP1; the disease is melanoma.