Because of the marked effects of K-Ras(V12) on Akt isoform expression, PANC-1/EGFP-K-Ras(V12), PANC-1/EGFP-expressing cells (Fig. 3), as well as H23 lung carcinoma cells, which express all three Akt isoforms (Fig. 4), were chosen for Akt depletion. This evidence concerns the gene AKT1 and lung carcinoma.