Because of the marked effects of K-Ras(V12) on Akt isoform expression, PANC-1/EGFP-K-Ras(V12), PANC-1/EGFP-expressing cells (Fig. 3), as well as H23 lung carcinoma cells, which express all three Akt isoforms (Fig. 4), were chosen for Akt depletion. The gene discussed is KRAS; the disease is lung carcinoma.