Based on qRT-PCR analysis conducted on 55 breast cancer patients and normal cases, it was observed that CACNG4, PKMYT1, EPYC, and CHRNA6 mRNA exhibited significantly higher expression levels in breast cancer tissues (BCT) compared to non-tumoral adjacent tissues (NTAT) (P < 0.0001). The gene discussed is PKMYT1; the disease is breast cancer.