LDHD and hepatocellular carcinoma: To further investigate the effect of LDHD on HCC proliferation, we down-regulated and up-regulated the expression level of LDHD in HepG2 and Huh7 cell lines by LDHD siRNA and GFP-tagged LDHD overexpression plasmid (GFP-LDHD) transfection, and verified the transfection efficiency using western blotting (Fig. 10A).