Differential expression of GPS1 in breast cancer was further analyzed using the TCGA-BRCA dataset, which showed that GPS1 was highly expressed in breast cancer tissues (5.88 ± 0.64) compared with normal tissues (5.24 ± 0.39) in the unpaired analysis (p < 0.001, Fig. 2A), and in breast cancer tissues (5.67 ± 0.68) compared with paracancerous tissues (5.24 ± 0.39) in the paired analysis (p < 0.001, Fig. 2B). This evidence concerns the gene GPS1 and breast cancer.