Expression of MTHFR in rectal adenocarcinoma by stages 2 and 3 compared to normal tissue showed a statistical significant difference (normal vs. stage 2, p = 0.003; normal vs. stage 3; p = 0.001; normal vs. stage 4, p = 0.001) and low expression correlated with higher cancer stages (Figure 1B; stage 1 vs. stage 3, p = 0.020; stage 1 vs. stage 4, p = 0.050). This evidence concerns the gene MTHFR and cancer.