MTHFR and rectum adenocarcinoma: This was confirmed by comparing the MTHFR expression profile of the left colon (Figure 2A) and rectal adenocarcinoma (Figure 1B) MTHFR gene expression of 138 individuals (Normal N = 20; Adjacent N = 59 and Tumor N = 59) confirmed that tumor tissue had overall lower MTHFR expression compared to adjacent and normal tissue, respectively.