Our metabolic analysis of OTUD3-knockout and wild-type cells also demonstrated significant increases in various metabolite levels in the former, including succinate, reduced glutathione, essential amino acids, nucleotide-related metabolites, etc. These metabolites contribute to aerobic glycolysis (the Warburg effect), oxidative stress response, protein synthesis, transcription, and other processes that support tumor cell growth and proliferation. The gene discussed is OTUD3; the disease is neoplasm.