To further elucidate the role of PVT1 in GBM, we conducted transcriptome sequencing on U251 cells with PVT1 stably knocked down, employing a criterion of |log2 (Fold Change)| > 1 and padj < 0.05 to select differentially expressed genes (Figure 4A). This evidence concerns the gene PVT1 and glioblastoma.