The venn diagrams were used to identify important hub genes, and combined with key genes enriched in the "pancreatic secretion pathway", various algorithms and screening results suggested that Prss1 and Cpa1 were important Hub gene, as shown in Fig. 7F. Therefore, it is suggested that the two genes play an important role in the mechanism of cadmium-induced cognitive impairment in F1 rats. This evidence concerns the gene PRSS1 and Cognitive impairment.