To investigate the role of EGR1 in HCC, we conducted an analysis of EGR1 protein expression in both normal liver cell line and HCC cell lines. Our findings revealed that HCC cell lines exhibited a significantly lower protein and mRNA expression of EGR1 compared to normal liver cell line (MIHA) (Fig. 2A and Fig. S2A). Subsequently, we utilized CRISPR/Cas9 technology to generate two single clones of EGR1 knockout (KO) cell lines in MHCC97H cells, while EGR1 expression was silenced in HCCLM3 cells using EGR1 siRNAs (Fig. 2B). The gene discussed is EGR1; the disease is hepatocellular carcinoma.