So far, the effects of the dual GIP/GLP-1 receptor agonist, TIR, on markers of neuronal growth (CREB and BDNF levels), apoptosis (BAX/Bcl2 ratio) differentiation, (pAkt, MAP2, GAP43, and AGBL4) and insulin resistance (GLUT1, GLUT4, GLUT3 and SORBS1) in human neuroblastoma cell line (SHSY5Y) exposed to normal (NG) and high concentration of glucose (HG) for 7 days were investigated. The gene discussed is CREB1; the disease is neuroblastoma.