SPARC and central nervous system cancer: ECM proteins have been reported to influence cell proliferation, adhesion, and migration [38] with additional studies suggesting that upregulation of SPARC could promote glioma invasion by altering the tumor microenvironment [39] We therefore further interrogated an additional 16 tumor-enriched ECM genes in our filtered list by evaluating the differential fold change in long-read count between tumor and adjacent comparator tissue sample.