For protein changes of PD-L1, 15 μM of acacetin in vitro (Fig. 4C) and 20 mg/kg of acacetin in vivo (Fig. 4D) drastically inhibited the protein levels of PD-L1 of NSCLC cells and A549-xenografted tumor tissues, respectively. This evidence concerns the gene CD274 and non-small cell lung carcinoma.