RALA and obesity due to melanocortin 4 receptor deficiency: RNA sequencing (RNA-seq) analysis from isolated mature adipocytes derived from control and HFD-fed mice28 revealed that Rala expression is significantly upregulated in adipocytes from epididymal WAT (eWAT) and iWAT during obesity development, whereas Ralgapa2 expression is downregulated (Fig. 1a,b).