To further probe the role of TRERNA1 on radiation tolerance of NSCLC cells, we transfected TRERNA1 siRNA into A549 cells with relative high TRERNA1 expression (si‐TRERNA1 group) to silence TRERNA1 expression and transfected TRERNA1 into HCC827 cells with relative low TRERNA1 expression (oe‐TRERNA1 group) to overexpress TRERNA1. This evidence concerns the gene TRERNA1 and non-small cell lung carcinoma.