TBK1 and IKKε were spatially separated from VP35 upon infection by EBOV trVLPs (Figure 1C, E), and IRF3 itself was demonstrated not to interact with VP35 and NP (Figure 5—figure supplement 1A, B), implying that other IRF3-interacting proteins might be involved in IRF3 sequestration in IBs upon viral infection. Here, TBK1 is linked to viral infectious disease.