Notably, free radicals have been involved in CIS-induced multiple organ injury, as reactive oxygen species (ROS) can activate enormous output of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-1β and IL-6 (Miller et al. 2010; Barabas et al. 2008; El-Awady el et al. 2011). This evidence concerns the gene TNF and in situ carcinoma.