TESMIN and hepatocellular carcinoma: Turning to functional outcomes, CCK-8 assays reveal that tesmin knockdown resulted in a notable reduction in cell proliferation viability in both Hep 3B2.1-7 and HepG2 cell lines when compared to control cells treated with scrambled shRNA, underscoring the role of tesmin in promoting cell proliferation in the context of HCC (Figure 3(c and d)).