We targeted six genetic loci in two genes (ASH1L1, KCNQ2) that are relevant to autism spectrum disorder and epilepsy [25], [26], [27], [28] for repair of disease-related variants in participant iPSC lines and one genetic locus in the third gene (GNAQ) linked to Sturge-Weber syndrome [29] for insertion of a disease-related variant in a control iPSC line by HDR (Table 2). The gene discussed is ASH1L; the disease is autism spectrum disorder.