Our results showed that although the content of UCP1 in the EA group was up-regulated, the content of UCP1 in the BAT of SAMP8 mice was still much lower than that in SAMR1 mice (P< 0.01), which also suggests that thermal regulation and metabolic disorders in SAMP8 mice may be related to low BAT activity. Here, UCP1 is linked to metabolic disease.