The current study elucidated the role of the RNA-binding protein LARP1 in GC cell cycle progression and metastatic behavior using in vitro cell experiments and demonstrated that LARP1 knockdown promoted cell apoptosis, arrested the cell cycle at the G2/M phase, and suppressed cell viability, migration, invasion, and colony formation in GC cells. This evidence concerns the gene LARP1 and gastric cancer.