Autoantibody profiling has evolved and expanded sequentially with time, consisting of antinuclear antibody (ANA) by indirect immunofluorescence (IIF), myositis associated antibodies (anti RNP/Sm, anti Ro-60, anti La, anti Ro-52) and anti Jo-1 (myositis specific antibody) as part of EUROIMMUN ANA profile-3 immunoblot assay. Here, RO60 is linked to myositis disease.