Despite the large interindividual variability of TF mRNA levels observed in nonneoplastic tissues, even when considering HCC and CC separately, when −ΔΔCtN values were compared between patients with high TF mRNA levels and patients with low TF mRNA levels in cancer tissue, the differences were not statistically significant (0.48 vs −0.41, respectively; P = .193 by t-test). This evidence concerns the gene TF and cholangiocarcinoma.