In order to explore the relationship between the up-regulation of lyst expression after infection and the secretion of C. burnetii effectors, THP-1 cells were differentiated and infected with C. burnetii pJb-TEM1-CvpE [positive control, a T4SS effector (Lührmann et al., 2010; Larson et al., 2015)], C. burnetii pJb-TEM1-Cbu1754 [negative control, a structural protein (Valdivia et al., 2014; Steiner et al., 2021)], dotA::Tn pJb-TEM1-CvpE or icmD::Tn pJb-TEM1-CvpE and subjected to TEM translocation assays according to the LiveBLAzerTM FRET—B/G Loading Kit. The gene discussed is CD248; the disease is infection.