POLE and colorectal cancer: Other relevant PTEN mutations either unique or more frequent in Groups 2 and 3 were E7*, R15K, E299*/X, R142W, F154L/V, R173C, F341V/C; some of these have been previously observed in POLE MSS high TMB colorectal cancers (49) and their nucleotide context is a POLE mutation signature (ref. 40; Supplementary Table S1).