Among 15 pathways in cancer, the differential mutation pathways between the MSI-H and MSS groups included the DDR (98% vs. 60%), SWI/SNF (96% vs. 33%), HRD (81% vs. 22%), CellCycle (79% vs. 62%), and PI3K (75% vs. 24%) (Additional File 1: Table S6, Fig. 1A). This evidence concerns the gene PIK3CA and cancer.